Measure nuclear

Measure the intensity in one or more chanels in the nuclei of cells

To measure nuclear intensity, choose the option Measure nuclear staining in the main pipeline interface

To measure the intensity in the nuclei, they must have been segmented before. If you haven't done it yet, go to Get nuclei step first.

This step allows to measure the nuclear intensity of a chosen staining inside each segmented nucleus. Choose the channel to measure and click on Measure to launch the computation. The measure can take time.

Once the measure is done, you can visualize the table of measurement by clicking Show measures table. A new window will pop up with the list of nucleus and their label, along with the measures in the selected channel.

Clicking Save and stop will save these results for all nuclei associated to one cell in the imagename_results.csv result file. However, if you want to save the nuclear intensity in ALL nuclei (not only the ones that are linked to a cell), you can click on Save all nuclei measures. This will create a new result file in the results folder, called imagename_nuclei.csv that contains the displayed table data.